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wizard sv gel and pcr clean-up system purification kit  (Promega)

 
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    Structured Review

    Promega wizard sv gel and pcr clean-up system purification kit
    Wizard Sv Gel And Pcr Clean Up System Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wizard+sv+gel+and+pcr+clean-up+system+kit/wizard+genomic+dna+purification+kit/pmc12280279-78-8-7
    Average 90 stars, based on 1 article reviews
    wizard sv gel and pcr clean-up system purification kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: New Insights into the Sex Chromosome Evolution of the Common Barker Frog Species Complex (Anura, Leptodactylidae) Inferred from Its Satellite DNA Content.
    Article Snippet: We used the RepeatMasker program (https://www.repeatmasker.org/; acessed on 20 January 2025) [43] to characterize the sequences included in the custom library generated from the RepeatExplorer analysis.

    Article Title: Prolonged fasting and glucocorticoid exposure drive dynamic DNA methylation in elephant seals.
    Article Snippet: DNA was purified using a WizardR SV Gel and PCR Clean-Up System kit (Promega) and quantified using a NanoDrop spectrophotometer (Nanodrop Technologies, Wilmington, DE) and a Qubit fluorometer (Invitrogen).

    Purification:

    Article Title: Less is more: the lack of autoinducer-2-dependent quorum sensing promotes competitive fitness of Escherichia coli strain 83972
    Article Snippet: .. The gels were run at 110–130 V for 30–60 min. Amplicons were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) or the Wizard SV Gel and PCR Clean-Up System kit (Promega, Walldorf, Germany). .. Plasmids were isolated using the NucleoSpin Plasmid Mini kit (Macherey-Nagel).

    Article Title: Microbiome and Resistome in Poultry Litter-Fertilized and Unfertilized Agricultural Soils.
    Article Snippet: .. The PCR products were then purified with the Wizard SV Gel and PCR Clean-Up System Kit (Promega Corporation, Madison, WI, USA). .. The concentration of the purified samples was measured with a Qubit 4 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Molecular detection of hemoplasmas in domestic cats from different Brazilian regions
    Article Snippet: The data generated was stored in the Image lab program, BioRad. .. The amplicons obtained in the snPCR and PCR assays for the 16S rRNA and 23S rRNA genes from hemoplasmas were purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega, Madison, Wisconsin, USA). ..

    Article Title: Yellow Fever Virus in Mosquitoes from Rainforest Bordering Manaus, Brazil, 2022
    Article Snippet: .. The fragments of interest were excised from the agarose and purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega, USA). .. Purified products were subjected to sequencing reactions using the BigDye kit (Applied Biosystems, USA) and reconstituted in formamide.

    Article Title: Phylodynamic of Tomato Brown Rugose Fruit Virus and Tomato Chlorosis Virus, Two Emergent Viruses in Mixed Infections in Argentina
    Article Snippet: For reverse transcription, 2 μL of Primer A (50 μM) (5′-GTTTCCCAGTCACGATC-N9-3′) was used with SuperScript III Reverse Transcriptase (Thermo Fisher Scientific Inc. Whaltam, MA, USA). .. Following second-strand cDNA synthesis using Klenow polymerase (New England Biolabs, Ipswich, MA, USA), the products were purified with the Wizard SV Gel and PCR Clean-Up System Kit (Promega Corp, Madison, WI, USA), following the manufacturer’s instructions. .. The purified DNA products were then amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA) in two successive rounds of PCR.

    Article Title: Phylodynamic of Tomato Brown Rugose Fruit Virus and Tomato Chlorosis Virus, Two Emergent Viruses in Mixed Infections in Argentina
    Article Snippet: .. In the second round of PCR, Primer B (5′-GTTTCCCAGTCACGATC-3′) was used, with the following conditions: 94 °C for 3 min, 35 amplification cycles (94 °C for 30 s, 54 °C for 30 s, and 72 °C for 2 min), and a final extension at 72 °C for 5 min. PCR products were purified using the Wizard SV Gel and PCR Clean-Up System Kit (Promega Corp, Madison, WI, USA). .. Samples were paired-end sequenced on the Illumina platform (read length 150 bp) at Novogene Bioinformatics Technology Co., Ltd.

    Polymerase Chain Reaction:

    Article Title: Less is more: the lack of autoinducer-2-dependent quorum sensing promotes competitive fitness of Escherichia coli strain 83972
    Article Snippet: .. The gels were run at 110–130 V for 30–60 min. Amplicons were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) or the Wizard SV Gel and PCR Clean-Up System kit (Promega, Walldorf, Germany). .. Plasmids were isolated using the NucleoSpin Plasmid Mini kit (Macherey-Nagel).

    Article Title: Microbiome and Resistome in Poultry Litter-Fertilized and Unfertilized Agricultural Soils.
    Article Snippet: .. The PCR products were then purified with the Wizard SV Gel and PCR Clean-Up System Kit (Promega Corporation, Madison, WI, USA). .. The concentration of the purified samples was measured with a Qubit 4 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Molecular detection of hemoplasmas in domestic cats from different Brazilian regions
    Article Snippet: The data generated was stored in the Image lab program, BioRad. .. The amplicons obtained in the snPCR and PCR assays for the 16S rRNA and 23S rRNA genes from hemoplasmas were purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega, Madison, Wisconsin, USA). ..

    Article Title: Yellow Fever Virus in Mosquitoes from Rainforest Bordering Manaus, Brazil, 2022
    Article Snippet: .. The fragments of interest were excised from the agarose and purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega, USA). .. Purified products were subjected to sequencing reactions using the BigDye kit (Applied Biosystems, USA) and reconstituted in formamide.

    Article Title: Phylodynamic of Tomato Brown Rugose Fruit Virus and Tomato Chlorosis Virus, Two Emergent Viruses in Mixed Infections in Argentina
    Article Snippet: For reverse transcription, 2 μL of Primer A (50 μM) (5′-GTTTCCCAGTCACGATC-N9-3′) was used with SuperScript III Reverse Transcriptase (Thermo Fisher Scientific Inc. Whaltam, MA, USA). .. Following second-strand cDNA synthesis using Klenow polymerase (New England Biolabs, Ipswich, MA, USA), the products were purified with the Wizard SV Gel and PCR Clean-Up System Kit (Promega Corp, Madison, WI, USA), following the manufacturer’s instructions. .. The purified DNA products were then amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA) in two successive rounds of PCR.

    Article Title: Phylodynamic of Tomato Brown Rugose Fruit Virus and Tomato Chlorosis Virus, Two Emergent Viruses in Mixed Infections in Argentina
    Article Snippet: .. In the second round of PCR, Primer B (5′-GTTTCCCAGTCACGATC-3′) was used, with the following conditions: 94 °C for 3 min, 35 amplification cycles (94 °C for 30 s, 54 °C for 30 s, and 72 °C for 2 min), and a final extension at 72 °C for 5 min. PCR products were purified using the Wizard SV Gel and PCR Clean-Up System Kit (Promega Corp, Madison, WI, USA). .. Samples were paired-end sequenced on the Illumina platform (read length 150 bp) at Novogene Bioinformatics Technology Co., Ltd.

    cDNA Synthesis:

    Article Title: Phylodynamic of Tomato Brown Rugose Fruit Virus and Tomato Chlorosis Virus, Two Emergent Viruses in Mixed Infections in Argentina
    Article Snippet: For reverse transcription, 2 μL of Primer A (50 μM) (5′-GTTTCCCAGTCACGATC-N9-3′) was used with SuperScript III Reverse Transcriptase (Thermo Fisher Scientific Inc. Whaltam, MA, USA). .. Following second-strand cDNA synthesis using Klenow polymerase (New England Biolabs, Ipswich, MA, USA), the products were purified with the Wizard SV Gel and PCR Clean-Up System Kit (Promega Corp, Madison, WI, USA), following the manufacturer’s instructions. .. The purified DNA products were then amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA) in two successive rounds of PCR.

    Amplification:

    Article Title: Phylodynamic of Tomato Brown Rugose Fruit Virus and Tomato Chlorosis Virus, Two Emergent Viruses in Mixed Infections in Argentina
    Article Snippet: .. In the second round of PCR, Primer B (5′-GTTTCCCAGTCACGATC-3′) was used, with the following conditions: 94 °C for 3 min, 35 amplification cycles (94 °C for 30 s, 54 °C for 30 s, and 72 °C for 2 min), and a final extension at 72 °C for 5 min. PCR products were purified using the Wizard SV Gel and PCR Clean-Up System Kit (Promega Corp, Madison, WI, USA). .. Samples were paired-end sequenced on the Illumina platform (read length 150 bp) at Novogene Bioinformatics Technology Co., Ltd.



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